#2 - M6: ISP-4

Name:M6: ISP-4
Content:Solution I:
Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.

Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see medium 609) 1.0 ml

The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.