#1 - M1: ISP-3

Name:M1: ISP-3
Content:

 

 

Oatmeal (Quaker White Oats)

20.0

g

Agar (Difco)

18.0

g

Distilled water

1000.0

ml



Adjust pH to 7.2. Cook or steam 20.0 g in 1000.0 ml distilled water for 20 min. Filter through cheese cloth. Add 18.0 g agar and make up to 1000.0 ml. Add 1 ml of trace salts solution (see below).

Trace salt solution:

 

FeSO4 x 7 H2O

0.1

g

MnCl2 x 4 H2O

0.1

g

ZnSO4 x 7 H2O

0.1

g

Distilled water

100.0

ml